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Abstract We present results of a multiwavelength analysis of SDSS J025214.67−002813.7, a system that has been previously classified as a binary active galactic nucleus (AGN) candidate based on periodic signals detected in the optical light curves. We use available radio−X-ray observations of the system to investigate the true accretion nature. Analyzing new observations from XMM-Newton and NuSTAR, we characterize the X-ray emission and search for evidence of circumbinary accretion. Although the 0.5–10 keV spectrum shows evidence of an additional soft emission component, possibly due to extended emission from hot nuclear gas, we find the spectral shape is consistent with that of a single AGN. Compiling a full multiwavelength spectral energy distribution (SED), we also search for signs of circumbinary accretion, such as a “notch” in the continuum due to the presence of minidisks. We find that the radio–optical emission agrees with the SED of a standard, radio-quiet, AGN; however, there is a large deficit in emission blueward of ∼1400 Å. Although this deficit in emission can plausibly be attributed to a binary AGN system, we find that the SED of SDSS J0252−0028 is better explained by emission from a reddened, single AGN. However, future studies of the expected hard X-ray emission associated with binary AGNs (especially in the unequal-mass regime) will allow for more rigorous analyses of the binary AGN hypothesis. 

Helicases play key roles in genome maintenance, yet it remains elusive how these enzymes change conformations and how transitions between different conformational states regulate nucleic acid reshaping. Here, we developed a computational technique combining structural bioinformatics approaches and atomic-level free-energy simulations to characterize how the Escherichia coli DNA repair enzyme UvrD changes its conformation at the fork junction to switch its function from unwinding to rezipping DNA. The lowest free-energy path shows that UvrD opens the interface between two domains, allowing the bound ssDNA to escape. The simulation results predict a key metastable 'tilted' state during ssDNA strand switching. By simulating FRET distributions with fluorophores attached to UvrD, we show that the new state is supported quantitatively by single-molecule measurements. The present study deciphers key elements for the 'hyper-helicase' behavior of a mutant and provides an effective framework to characterize directly structure-function relationships in molecular machines.